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Protocol - Basal Metabolic and Resting Metabolic Rates

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Protocol Name from Source:

This section will be completed when reviewed by an Expert Review Panel.

Availability:

Publicly available

Description:

A 30- to 40-minute process (not including the 30-minute rest period required prior to the start of the measure) for measuring basal (BMR) and resting metabolic rates (RMR) using indirect calorimetry, a measure of energy expenditure. By definition, BMR is measured in individuals in a reclining position, upon awakening in the morning after a 12-hour fast under thermoneutral conditions. The postprandial period is relaxed to 2-4 hours and the time of day uncontrolled for RMR measurements. Using an indirect calorimeter, investigators measure the concentrations of oxygen and carbon dioxide in air streams entering and exiting a clear plastic hood placed over the participant’s head. Oxygen consumption and carbon dioxide production are calculated from the change in concentration and flow rate.

Protocol:

1. This protocol describes the commonly used metabolic cart equipped with a canopy hood for measurement of BMR or RMR

2. Ensure that the equipment has been properly calibrated at the start of each day.

3. Ensure that the equipment is turned on and warmed up for at least 30 minutes prior to using it with a participant.

4. Ask the participant to lie down and rest quietly for about 30 minutes.

5. If the participant feels cold, offer him/her a blanket. If the participant feels hot, alter the environment to insure that he/she does not sweat.

6. Check that the monitor is in canopy mode. Change if needed (This may differ between instruments).

7. Check that the monitor is in the artifact suppression mode with a 10 min start delay. (This may differ between instruments).

8. Check that the hoses from the hood to the metabolic monitor are connected and the unit is turned on.

9. Perform a calibration of the metabolic monitor, as needed.

10. After the initial 30 minute rest period, measure the resting metabolic rate as per instrument instructions.

11. The printer should be reporting data on a minute by minute basis. If it is not printing, check the connections, printer power, or see the PRINTER SETUP.

12. Proceed with the measurement for 30-40 min.

• The technician must remain with the participant - monitoring gas flow alarms and visually checking for labored breathing to insure that gas flow does not fail.

• The participant must remain at rest but not sleep. We recommend that you speak to the participant if you feel it is necessary to keep him/her from falling asleep. If that approach is not effective, then a second attempt could be made by a gentle "nudge." It is important that staff are careful not to startle participants in doing this; however as this could cause an increased heart rate, thereby affecting the test. Participant should always be warned verbally in advance of a physical contact.

• Use of classical music and a relaxing poster over the bed are acceptable methods for keeping participants calm during the indirect calorimetry test. However, please do not use videos or any other strategies for relaxation

• The participant must not talk, except when necessary to communicate a potential problem. If the participant does talk, lift their arms to scratch an itch, shift their weight to prevent stiffness etc., indicate the time and movement on the printout using a pen or pencil.

• Confirm that the participant is still thermally comfortable.

• If the participant has to get up because he/she needs to use the bathroom, then the measurement can be terminated, but the participant will need to start over. The measurement sequence (i.e., steps 3-13) needs to be repeated beginning with a 10 min rest in place of the 30 min called for in the basic protocol.

13. At 30 minutes, check the display data printout for a stable reading (steady state).

14. End the measurement.

15. Obtain the output data from the metabolic cart (time, VO2, VCO2, RQ, Energy Expenditure). Save the readings on a disk or other electronic format. If electronic output not available, staff will enter output onto Excel spreadsheet.

16. Remove the hood from over the participant’s head.

17. Ask the participant to sit upright.

18. Help the participants to their feet and be sure that they are steady. Remember that they have fasted and there is a small risk of hypoglycemia.

19. Sanitize the canopy per local procedures.

Note: The primary safety concern is that airflow through the hood is maintained while the hood is in place over the participant’s head. Loss of flow due to a rare failure of the fan in the metabolic cart or due to a loose hose will cause discomfort and in an extreme case may cause asphyxiation. Although an alarm will sound if the unit does not detect breathing, the metabolic cart technician should remain with the participant throughout the measurement. Care should also be exercised when the participant stands-up after the measurement should dizziness develop secondary to the fast.

Post-Indirect Calorimetry Quality Check

After completion of the indirect calorimetry test, complete a quality check on the following:

1. The printout/electronic record is legible. If not, correct problem and reprint.

2. The average RQ is between 0.75 and 0.9. Values outside of this range may indicate that the participant fasted longer than 15 h (<0.75), ate within the last 6 h (>0.93), or hyperventilated during the measurement (>0.93). Other possible explanations are very high fat diets (<0.75), a weight loss diet (<0.75) or very high carbohydrate diets (>0.93). If the participant admits to a recent meal, reschedule the test.

Check that the coefficient of variation is less than 10%. Possible explanations are excessive participant movement, irregular breathing pattern, failure to suppress the first 10 min of the measurement, or instrument maintenance problems. If the first 10 min of the measurement were not deleted, manually calculate the average and SD without the first 10 min. If the revised coefficient of variation is less than 10%, record these values. If not, repeat the measurement of resting metabolic rate.

Personnel and Training Required

Training of technician to operate the metabolic cart

Equipment Needs

Indirect calorimeter, referred to as a metabolic cart

Requirements

Requirement CategoryRequired
Average time of greater than 15 minutes in an unaffected individualYes
Major equipmentYes
Specialized requirements for biospecimen collectionNo
Specialized trainingYes

Mode of Administration

Self-administered

Life Stage:

Child, 5 years or older, Adolescent, Adult, Senior, Pregnancy

Specific Instructions:

Participants who have self-reported claustrophobia, which would cause them to become anxious when the plastic hood is placed over their head, should be excluded from taking part in the indirect calorimetry procedures. The procedures require that the participant lie quietly for 30 minutes; anxiety caused by claustrophobia would produce inaccurate test results. If participant fasted for 12 hours, but had caffeine within 2-4 hours of the visit, then the indirect calorimetry procedure should be rescheduled. If it has been more than 2-4 hours since consumption of caffeine, the participant may complete the indirect calorimetry procedures because the caffeine has likely been metabolized.

Research Domain Information

Release Date:

September 9, 2015

Definition

This measures basal metabolic rate (BMR) and resting metabolic rate (RMR) using indirect calorimetry (a measurement of energy expenditure based on oxygen consumption and carbon dioxide production).

Purpose

Obesity arises from energy imbalance. Measurement of energy expenditure and fuel utilization is fundamental to understanding the development of obesity.

Selection Rationale

Obesity arises from energy imbalance. Measurement of energy expenditure and fuel utilization is fundamental to understanding the development of obesity. Indirect calorimetry is a widely used method for capturing RMR and BMR.

Language

English

Standards

StandardNameIDSource
Common Data Elements (CDE)Obesity Metabolic Rate Quantification Assessment Score4994115CDE Browser

Process and Review

This section will be completed when reviewed by an Expert Review Panel.

Source

Hispanic Community Health Study (HCHS)/Study of Latinos (SOL). Nutritional and Physical Activity Assessment Study (SOLNAS). 2012. Section 6.4.3.8.

General References

Sorlie, P. D., Avilés-Santa, L. M., Wassertheil-Smoller, S., Kaplan, R. C., Daviglus, M. L., Giachello, A. L., Schneiderman, N., Raij, L., Talavera, G., Allison, M., LaVange, L., Chambless, L. E., & Heiss, G. (2010). Design and implementation of the Hispanic Community Health Study / Study of Latinos. Annals of Epidemiology, 20(8), 629-641.

Protocol ID:

230301

Variables:

Export Variables
Variable NameVariable IDVariable DescriptionVersiondbGaP Mapping
PX230301_BasalandResting_MetabolicRates_Initial30Min_RestingPX230301010000After the initial 30 minute rest period, measure the resting metabolic rate as per instrument instructions. ______________4N/A
PX230301_BasalandResting_MetabolicRates_CartData_TimePX230301020000Obtain the output data from the metabolic cart: Time - ____________4N/A
PX230301_BasalandResting_MetabolicRates_CartData_VO2PX230301030000Obtain the output data from the metabolic cart: VO2 - ____________4N/A
PX230301_BasalandResting_MetabolicRates_CartData_VCO2PX230301040000Obtain the output data from the metabolic cart: VCO2 - ____________4N/A
PX230301_BasalandResting_MetabolicRates_CartData_RQPX230301050000Obtain the output data from the metabolic cart: RQ - ____________4N/A
PX230301_BasalandResting_MetabolicRates_CartData_EnergyExpenditurePX230301060000Obtain the output data from the metabolic cart: Energy Expenditure - ____________4N/A
PX230301_BasalandResting_MetabolicRates_QualityCheck_LegiblePX230301070000The printout/electronic record is legible. If not, correct problem and reprint.4N/A
PX230301_BasalandResting_MetabolicRates_QualityCheck_RQValuePX230301080000RQ Value: _______________ (The average RQ is between 0.75 and 0.9)4N/A
PX230301_BasalandResting_MetabolicRates_QualityCheck_CoefficientPX230301090000Check that the coefficient of variation is less than 10%. Possible explanations are excessive participant movement, irregular breathing pattern, failure to suppress the first 10 min of the measurement, or instrument maintenance problems. If the first 10 min of the measurement were not deleted, manually calculate the average and SD without the first 10 min. If the revised coefficient of variation is less than 10%, record these values. If not, repeat the measurement of resting metabolic rate.4N/A